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Although the existing body of research generally supports the notion that e-cigarettes are less harmful than regular/combustible cigarettes, attributed primarily to the lack of combustion and relatively fewer toxic compounds in e-cigarettes aerosols compared to cigarette smoke [4, 5], this does not mean that e-cigarettes are void of risk

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The vapor produced contains harmful substances like nicotine, which is addictive, and other chemicals that can damage the lungs and cardiovascular system, similar to the effects of smoking

Total cellular RNA was extracted using TRI-Reagent (Sigma) according to the manufacturer's instructions