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RNA extraction and RT-qPCR analysis Sample total RNA was extracted and reverse transcribed, and RT-qPCR was performed using a Plant Total RNA Isolation Kit (Foregene, Chengdu, China), HiScript II Q RT SuperMix for qPCR (Vazyme, Nanjing, China), and ChamQ Blue Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China), respectively, and the specific procedures were followed as we described previously [50]

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Whereas, for others, the priority is clear: nicotine without tobacco

A recent study looked at trends in childrens emergency department visits for asthma in states that passed indoor smoking bans in public places and workplaces

1 2 "Smoking and carcinoma of the lung