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Medium Hepatocyte maintenance medium (HMM) is DMEM/F12 (Gibco) supplemented with 0.544 mg/L ZnCl 2 (Sinopharm), 0.75 mg/L ZnSO 4 7H 2 O (Sinopharm), 0.2 mg/L CuSO 4 5H 2 O (Sinopharm), 0.025 mg/L MnSO 4 (Sinopharm), 2 g/L Bovine serum albumin (Sigma-Aldrich), 2 g/L Galactose (Sigma-Aldrich), 0.1 g/L Ornithine, 0.03 g/L Proline, 0.61 g/L Nicotinamide, 1 Insulin-transferrin-sodium selenite media supplement (Sigma-Aldrich), 40 ng/mL TGF (Peprotech), 40 ng/mL EGF (Peprotech), 10 M dexamethasone, 10 M Y-27632 (MCE), 0.5 M A-83-01 (Tocris), 3 M CHIR99021 (Sigma-Aldrich)

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