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Nicotine, an alkaloid found in tobacco leaves, is a colorless liquid that turns brown when it combines with oxygen and is the substance that causes physiological dependence

This is only visible on the inside of the cigarette machine, do not open it if your machine is still under warranty

The bacteria involved in the fermentation process interact with natural alkaloids in the tobacco, converting them into incredibly high concentrations of Tobacco-Specific Nitrosamines

Toxicol Appl Pharmacol 199(3):193209 Tantcheva-Por I, Zaigler M, Rietbrock S, Fuhr U (1999) Estimation of cytochrome P-450 CYP1A2 activity in 863 healthy Caucasians using a saliva-based caffeine test

A Markov model to estimate population-level patterns of cigarette and e-cigarette use