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These substances interfere with normal cellular processes needed for healing: namely fibroblast activity, collagen formation, tissue regeneration

While the study did not assess the levels of urine cotinine or carboxyhemoglobin, we selected CO as a biological marker because it is simple and quick to measure, less expensive than a battery of laboratory tests, and noninvasive, making it more agreeable to the subjects as reported by Middleton et al

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Over time, these traditional curing barns have fallen into disuse, in favor of more modern methods of curing tobacco