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Locus-specific DNA methylation Bisulfite pyrosequencing ( Locus-independent global DNA methylation Bisulfite pyrosequencing of repetitive DNA elements ( ELISA-based methylation assay ( [ 3 H]-methyl acceptance assay (Terry et al., 2008): Relies on SssI prokaryotic methylase to incorporate [ 3 H]-labeled methyl groups at all unmethylated CpGs