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Symptoms include: Flaccid (non-erect) penis Decreased sexual desire Trouble keeping an erection Causes of Erectile Dysfunction Some causes of erectile dysfunction are caused by factors that are out of the patients control, while other factors are modifiable

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RT-qPCR The total RNA of cultured epithelial cell lines was extracted by using TRIzol (Invitrogen, CA, USA) reagent according to the manufacturers protocol