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After cleavage with His-TEV to remove the His-MBP tag, the eluted fraction (SNX1-His-SUMO-SNX2a) was further loaded onto a Ni-NTA column and washed with buffer containing 20 mM HEPES (pH 7.4), 200 mM NaCl, and 40 mM imidazole to remove unbound samples
The aim of this study is to identify the behaviours and motivations of smokers and to analyse how to dispose of cigarettes after consumption and to quantify the amount of cigarette butts generated by the Romanian population, chosen because its tobacco consumption is very close to the average value of the European Union
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